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Poster De Conférence Année : 2023

UV and THz assisted APT analysis of a silica matrix for bio-particles embedding

Résumé

Unravelling the 3D structure and composition of biological macromolecules at nanoscale sheds light on their functions and interactions with other molecules. In such a context, Atom Probe Tomography (APT) is lately arousing interest because it provides three-dimensional compositional mapping with subnanometre resolution, adding high chemical sensitivity throughout the whole periodic table of elements [1].The development of the laser-assisted APT (La-APT) has extended the APT analysis from metallic materials to dielectric materials by combining the actions of a high DC field and an ultrashort laser pulse [2]. Recently, it has been shown that bio-molecules can be embedded in dielectric materials such as silica and analysed by La-APT [3]. In this work, we focus on the APT analysis of amorphous silica matrix samples, synthesized via a sol-gel process, a promising candidate for the development of an effective bio-particles embedding method, providing an acqueous-like environment for biomelecules, as demonstrated in previous works [3]. The specimen was analysed using two different APT setups, both operating under ultravacuum cryogenic conditions, but differing for laser illumination configurations: a La-APT shining UV radiation at λ=343 nm and a Terahertz-assisted APT, where the UV laser is replaced by a high field THz mono-cycle (λ=1-0.1mm, 200kV/cm amplitude, 500fs duration). Our objective is to find the optimal parameters (laser power, THz amplitude, DC field) to improve the accuracy of compositional analyses, thus to increase the signal to noise ratio and the mass resolution power of the mass spectra reported in Fig. 1 and Fig.2, for the La-APT and the THz-APT, respectively.
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Dates et versions

hal-04190588 , version 1 (29-08-2023)

Identifiants

  • HAL Id : hal-04190588 , version 1

Citer

Matteo de Tullio, Ivan Blum, Emmanuel Cadel, Laurence Chevalier, Gustav Eriksson, et al.. UV and THz assisted APT analysis of a silica matrix for bio-particles embedding. 18e colloque de la Société Française des Microscopies, Jul 2023, Rouen (France), France. ⟨hal-04190588⟩
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