In vitro effects of glyphosate-based herbicides and related adjuvants on primary culture of hemocytes from Haliotis tuberculata - Normandie Université Access content directly
Journal Articles Fish and Shellfish Immunology Year : 2020

In vitro effects of glyphosate-based herbicides and related adjuvants on primary culture of hemocytes from Haliotis tuberculata

Abstract

Glyphosate-based herbicides are among the most produced and widely-used herbicides. Studies have shown that commercial formulations and adjuvants may be more toxic to non-target organisms than the active ingredients alone, but the mechanisms of action of these chemicals remain unclear. The aim of this study was to investigate the in vitro effects of glyphosate, a commercial formulation and adjuvant alone using primary culture of hemocytes from the European abalone Haliotis tuberculata, a commonly farmed shellfish. Glyphosate was found to have negligible effects on viability, phagocytic activities and lysosome stability even with very high doses (i.e. 100 mg L−1). By contrast, greater effects on viability were observed for the commercial formulation and adjuvant alone, with EC50 values of 41.42 mg L−1 and 1.85 mg L−1, respectively. These results demonstrate that the toxic sublethal effects (i.e. phagocytic activity and destabilization of lysosomal membranes) of formulated glyphosate came from adjuvants and suggest they may be related to cell and organelle membrane destabilization.
Fichier principal
Vignette du fichier
S1050464820301455.pdf (818.9 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-02904691 , version 1 (22-08-2022)

Licence

Identifiers

Cite

Antoine Mottier, Antoine Serpentini, Lorna Dallas, Adèle James, Jean-Marc Lebel, et al.. In vitro effects of glyphosate-based herbicides and related adjuvants on primary culture of hemocytes from Haliotis tuberculata. Fish and Shellfish Immunology, 2020, 100, pp.1-8. ⟨10.1016/j.fsi.2020.02.058⟩. ⟨hal-02904691⟩
59 View
54 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More