N-Glycosylation of Plant Recombinant Pharmaceuticals - Normandie Université Access content directly
Book Sections Year : 2009

N-Glycosylation of Plant Recombinant Pharmaceuticals

Abstract

N-glycosylation is a maturation event necessary for the correct function, efficiency, and stability of a high number of biopharmaceuticals. This chapter presented here proposes various methods to determine whether, how, and where a plant pharmaceutical is N-glycosylated. These methods rely on blot detection with glycan-specific probes, specific deglycosylation of glycoproteins followed by mass spectrometry, N-glycan profile analysis, and glycopeptide identification by LC-MS.
No file

Dates and versions

hal-02128578 , version 1 (14-05-2019)

Identifiers

Cite

Muriel Bardor, Gleysin Cabrera, Johannes Stadlmann, Patrice Lerouge, José Cremata, et al.. N-Glycosylation of Plant Recombinant Pharmaceuticals. Loïc Faye; Véronique Gomord. Recombinant Proteins From Plants. Methods and Protocols, 483, Springer, pp.239-264, 2009, Methods in Molecular Biology, 978-1-58829-978-9. ⟨10.1007/978-1-59745-407-0_14⟩. ⟨hal-02128578⟩
14 View
1 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More