A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers - Normandie Université Access content directly
Journal Articles Bio-protocol Year : 2015

A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers

Abstract

The pollen tube, a fast tip-growing cell, is an excellent model to study membrane and cell wall biosynthesis. Here, we describe a simple protocol using an easy to use device to perform immunofluorescence labelling of pollen tube membrane and cell wall. The use of the NucleoSpin column to perform all the steps of the immunolabelling procedure results in obtaining more intact pollen tubes.
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Dates and versions

hal-01838518 , version 1 (05-09-2019)

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Marie Dumont, Céline Cataye, Arnaud Lehner, Eric Maréchal, Patrice P. Lerouge, et al.. A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers. Bio-protocol , 2015, 5 (12), pp.e1502. ⟨10.21769/BioProtoc.1502⟩. ⟨hal-01838518⟩
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