Laboratory network surveillance of equine diseases - Normandie Université
Communication Dans Un Congrès Année : 2024

Laboratory network surveillance of equine diseases

Résumé

Background: The French Network for Surveillance of Equine Diseases (RESPE) is an online veterinary reporting and information system created in 1999 for early detection of equine syndromes and nonnotifiable diseases in France. It aims to provide an early warning to all the equine industry stakeholders to implement immediate and collective management measures when necessary. To increase its surveillance coverage, the RESPE is developing a laboratory-based surveillance component, that centralises laboratory analysis results from a network of sentinel laboratories. Diagnostic laboratory results from different laboratories must be comparable and reliable to ensure effective epidemiological indicators for surveillance use. However, equine influenza, strangles, and equine herpesvirus-1 and -4 (EHV-1 and EHV-4) infections which pose significant risks to the equine industry, are non-notifiable diseases. Thus, diagnostic laboratory methods are not standardised, and each laboratory can develop its own test. Objectives: To compare the PCR diagnostic laboratory results for equine influenza, strangles, EHV-1 and EHV-4 infections obtained by different laboratories. Study design: In vitro studies. Methods: Interlaboratory comparisons (ILC) were performed to assess the capacity of participating laboratories in diagnosing the targeted diseases using their PCR methods. ILCs were organised in 2022 and 2023. These consisted of sending panels of samples of known status, i.e. containing or not containing targeted pathogen DNA or RNA. The results obtained by the laboratories using their own method on the blinded samples were compared with the results obtained by LABEO, RESPE's support laboratory. Results: Six laboratories participated in the ILCs. The percentage of participating laboratories that correctly identified the samples was 80% for equine influenza and EHV-1, and 100% for strangles and EHV-4. Main limitations: Samples were artificially contaminated and none were at the detection limit. Conclusions: These results highlighted the importance of ensuring the reliability and comparability of the laboratory diagnostic methods for effective surveillance of equine infectious diseases. Ethical animal research: Not required: quality improvement activity. Informed consent: Not applicable. Competing interests: None declared. Funding: French Institute of horse and riding (IFCE), Conseil Départemental du Calvados, Fonds EPERON, French Ministry in charge of Agriculture, Conseil Départemental de la Manche, National Horseracing Federation (FNCH).
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Dates et versions

hal-04724006 , version 1 (07-10-2024)

Identifiants

Citer

Coralie Lupo, Christine Fortier, Eléna Kokabi, Albertine Léon, Stéphane Pronost, et al.. Laboratory network surveillance of equine diseases. 12th International Equine Infectious Diseases Conference, Sep 2024, Deauville, France. pp.79 (n°125), ⟨10.1111/evj.14215⟩. ⟨hal-04724006⟩
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