Human rheumatoid synovial cells (HRSC) in culture express TGF-β receptors and are growth stimulated by the factor
Résumé
TGF-β (5 ng/ml) increased the proliferation rate of HRSC by 30 % after 96 h of treatment. One type of TGF-β binding system, with apparent Kd of 953 pM and a number of 29400 receptors/cell, was detected by Scatchard analysis of [125I]-TGF-β binding. Howewer, crosslinking experiments and SDS-PAGE separation showed five TGF-β binding proteins: 50, 70, 110, 140 and 400 kDa. We may suggest that the affinities of these receptors are too close to be revealed by-Scatchard plot. All together, the data suggest that TGF-β plays a role in the hyperplasia of RA synovial tissue.