Modulation of interleukin-1 receptor expression by transforming growth factor-β in cultured rabbit articular chondrocytes: analysis by reverse transcription-polymerase chain reaction - Normandie Université Accéder directement au contenu
Article Dans Une Revue Osteoarthritis and Cartilage Année : 1995

Modulation of interleukin-1 receptor expression by transforming growth factor-β in cultured rabbit articular chondrocytes: analysis by reverse transcription-polymerase chain reaction

Résumé

Interleukin-1 receptor type I (IL-1RI) expression in cultured rabbit articular chondrocytes (RAC) was studied by reverse transcription-polymerase chain reaction (RT-PCR). A cDNA probe specific for the rabbit IL-1RI gene was constructed using primers derived from the sequence data of the human, murine and chick receptors. Transforming growth factor-beta 1 (TGF beta-1) was shown to transiently increase the level of expected 900-bp PCR product at 1 h of incubation and decrease the expression at 48 and 72 h with no effect at 24 h. In receptor binding assays using [125I]-IL-1 alpha, TGF beta decreased IL-1R bioactivity at all time points. These results suggest that TGF beta-induced down-regulation of IL-1 RI could be responsible for its ability to antagonize the effect of IL-1 and that TGF beta may have a role in the repair of articular cartilage.

Dates et versions

hal-04225179 , version 1 (02-10-2023)

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Stéphane Pronost, Nadine Segond, Margaret Macro, Françoise Rédini, Hubert Penfornis, et al.. Modulation of interleukin-1 receptor expression by transforming growth factor-β in cultured rabbit articular chondrocytes: analysis by reverse transcription-polymerase chain reaction. Osteoarthritis and Cartilage, 1995, 3 (3), pp.147-155. ⟨10.1016/s1063-4584(05)80049-4⟩. ⟨hal-04225179⟩
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