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Article Dans Une Revue Journal of Clinical Microbiology Année : 1999

Restriction Fragment Length Polymorphisms of Virulence Plasmids in Rhodococcus equi

Shinji Takai
  • Fonction : Auteur
Masato Shoda
  • Fonction : Auteur
Yukako Sasaki
  • Fonction : Auteur
Shiro Tsubaki
  • Fonction : Auteur
Stéphane Pronost
Karim Rahal
  • Fonction : Auteur
Vivian Nicholson
  • Fonction : Auteur

Résumé

Virulent Rhodococcus equi , which is a well-known cause of pyogranulomatous pneumonia in foals, possesses a large plasmid encoding virulence-associated 15- to 17-kDa antigens. Foal and soil isolates from five countries—Argentina, Australia, Canada, France, and Japan—were investigated for the presence of 15- to 17-kDa antigens by colony blotting, using the monoclonal antibody 10G5, and the gene coding for 15- to 17-kDa antigens by PCR. Plasmid DNAs extracted from positive isolates were digested with restriction endonucleases Bam HI, Eco RI, Eco T22I, and Hin dIII, and the digestion patterns that resulted divided the plasmids of virulent isolates into five closely related types. Three of the five types had already been reported in Canadian and Japanese isolates, and the two new types had been found in French and Japanese isolates. Therefore, we tentatively designated these five types 85-kb type I (pREAT701), 85-kb type II (a new type), 87-kb type I ( Eco RI and Bam HI type 2 [V. M. Nicholson and J. F. Prescott, J. Clin. Microbiol. 35:738–740, 1997]), 87-kb type II (a new type), and 90-kb (pREL1) plasmids. The 85-kb type I plasmid was found in isolates from Argentina, Australia, Canada, and France. Plasmid 87-kb type I was isolated in specimens from Argentina, Canada, and France. The 85-kb type II plasmid appeared in isolates from France. On the other hand, plasmids 87-kb type II and 90-kb were found only in isolates from Japan. These results revealed geographic differences in the distribution of the virulence plasmids found in the five countries and suggested that the restriction fragment length polymorphism of virulence plasmids might be useful to elucidate the molecular epidemiology of virulent R. equi in the world.

Dates et versions

hal-04225119 , version 1 (02-10-2023)

Identifiants

Citer

Shinji Takai, Masato Shoda, Yukako Sasaki, Shiro Tsubaki, Guillaume Fortier, et al.. Restriction Fragment Length Polymorphisms of Virulence Plasmids in Rhodococcus equi. Journal of Clinical Microbiology, 1999, 37 (10), pp.3417-3420. ⟨10.1128/JCM.37.10.3417-3420.1999⟩. ⟨hal-04225119⟩

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