Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen - Normandie Université Accéder directement au contenu
Article Dans Une Revue Journal of Clinical Microbiology Année : 2011

Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen

Résumé

This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.
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hal-04223070 , version 1 (29-09-2023)

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Fabien Miszczak, Kathleen Shuck, Zhengchun Lu, Yun Young Go, Jianqiang Zhang, et al.. Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen. Journal of Clinical Microbiology, 2011, 49 (10), pp.3694-3696. ⟨10.1128/JCM.01187-11⟩. ⟨hal-04223070⟩

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