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Article Dans Une Revue (Data Paper) Biotechnology Letters Année : 2012

Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol

Résumé

Genetically-modified Enterococcus faecalis has a potential of survival and can be used in ethanolic fermentations. Fermentation profiles of E. faecalis JH2-2 were assessed using glucose and lactose as carbon sources. Deletion of lactate dehydrogenase (ldh) genes increased the ethanol production from 0.25 to 0.82 g/l, which was further increased to 0.96 g/l by the insertion of a pyruvate decarboxylase (pdc) gene (from Sarcina ventriculi or Clostridium acetobutylicum) in place ldh1. When grown on lactose, the pdcSv and pdcCa showed 13.6 and 17.6 U mg−1 of pdc specific activity, respectively. Highest activity (47 U mg−1) and ethanol concentration (2.3 g/l) were obtained with pdcCa using an expression plasmid. Formate and acetate were also produced in high quantities. Transcriptional analysis showed that aldehyde alcohol dehydrogenase gene was upregulated up to 16-fold. Further optimizations are required for higher ethanol production.

Dates et versions

hal-04158326 , version 1 (11-07-2023)

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Citer

N. Rana, S. Gente, A. Rincé, Y. Auffray, J. Laplace. Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol. Biotechnology Letters, 2012, 34 (9), pp.1651-1657. ⟨10.1007/s10529-012-0964-x⟩. ⟨hal-04158326⟩
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