Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol - Normandie Université Access content directly
Journal Articles (Data Paper) Biotechnology Letters Year : 2012

Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol

Abstract

Genetically-modified Enterococcus faecalis has a potential of survival and can be used in ethanolic fermentations. Fermentation profiles of E. faecalis JH2-2 were assessed using glucose and lactose as carbon sources. Deletion of lactate dehydrogenase (ldh) genes increased the ethanol production from 0.25 to 0.82 g/l, which was further increased to 0.96 g/l by the insertion of a pyruvate decarboxylase (pdc) gene (from Sarcina ventriculi or Clostridium acetobutylicum) in place ldh1. When grown on lactose, the pdcSv and pdcCa showed 13.6 and 17.6 U mg−1 of pdc specific activity, respectively. Highest activity (47 U mg−1) and ethanol concentration (2.3 g/l) were obtained with pdcCa using an expression plasmid. Formate and acetate were also produced in high quantities. Transcriptional analysis showed that aldehyde alcohol dehydrogenase gene was upregulated up to 16-fold. Further optimizations are required for higher ethanol production.

Dates and versions

hal-04158326 , version 1 (11-07-2023)

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N. Rana, S. Gente, A. Rincé, Y. Auffray, J. Laplace. Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol. Biotechnology Letters, 2012, 34 (9), pp.1651-1657. ⟨10.1007/s10529-012-0964-x⟩. ⟨hal-04158326⟩
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