Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus
Abstract
This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.