Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus

This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

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Médecine vétérinaire et santé animale Virologie

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https://normandie-univ.hal.science/hal-02946322

Soumis le : mardi 3 septembre 2024-21:43:56

Dernière modification le : lundi 16 septembre 2024-13:30:05

Dates et versions

hal-02946322 , version 1 (03-09-2024)

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Identifiants

HAL Id : hal-02946322 , version 1
DOI : 10.1128/JCM.01935-14
PUBMED : 25339390
PUBMEDCENTRAL : PMC4290937

Citer

Aymeric Hans, Delphine Gaudaire, Jean-Claude Manuguerra, Albertine Léon, Antoine Gessain, et al.. Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus. Journal of Clinical Microbiology, 2014, 53 (1), pp.287-291. ⟨10.1128/JCM.01935-14⟩. ⟨hal-02946322⟩

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