6-Azido d-galactose transfer to N-acetyl-d-glucosamine derivative using commercially available β-1,4-galactosyltransferase - Normandie Université Access content directly
Journal Articles Tetrahedron Letters Year : 2008

6-Azido d-galactose transfer to N-acetyl-d-glucosamine derivative using commercially available β-1,4-galactosyltransferase

Abstract

A new strategy to tag glycoproteins carrying terminal GlcNAc was developed using commercially available bovine β-1,4-galactosyltransferase (GalT) and UDP-6-azidogalactose. The azide function was then allowed to react via a biotinylated Staudinger–Bertozzi probe demonstrating the usefulness of such a procedure to tag any glycoprotein possessing a N-acetylglucosamine terminal residue from any type of cell lysate.

Dates and versions

hal-02384444 , version 1 (28-11-2019)

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Michaël Bosco, Sophie Le Gall, Christophe Rihouey, Samuel Couve-Bonnaire, Muriel Bardor, et al.. 6-Azido d-galactose transfer to N-acetyl-d-glucosamine derivative using commercially available β-1,4-galactosyltransferase. Tetrahedron Letters, 2008, 49 (14), pp.2294-2297. ⟨10.1016/j.tetlet.2008.02.018⟩. ⟨hal-02384444⟩
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