Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching - Normandie Université Access content directly
Journal Articles Molecular Plant-Microbe Interactions Year : 2019

Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching

A Chane
C Barbey
  • Function : Author
M Robert
  • Function : Author
A Merieau
  • Function : Author
Y Konto-Ghiorghi
A Beury-Cirou
  • Function : Author
M Feuilloley
  • Function : Author
M Patek
  • Function : Author
V Gobert
  • Function : Author

Abstract

Confocal laser-scanning microscopy was chosen to observe the colonization and damage caused by the soft rot Pectobacterium atrosepticum and the protection mediated by the biocontrol agent Rhodococcus erythropolis. We developed dual-color re- porter strains suited for monitoring quorum-sensing and quorum-quenching activities leading to maceration or bio- control, respectively. A constitutively expressed cyan or red fluorescent protein served as a cell tag for plant colonization, while an inducible expression reporter system based on the green fluorescent protein gene enabled the simultaneous re- cording of signaling molecule production, detection, or deg- radation. The dual-colored pathogen and biocontrol strains were used to coinoculate potato tubers. At cellular quorum, images revealed a strong pectobacterial quorum-sensing ac- tivity, especially at the plant cell walls, as well as a concomitant rhodococcal quorum-quenching response, at both the single- cell and microcolony levels. The generated biosensors appear to be promising and complementary tools useful for molecular and cellular studies of bacterial communication and interference.
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Dates and versions

hal-02356443 , version 1 (08-11-2019)

Identifiers

  • HAL Id : hal-02356443 , version 1

Cite

A Chane, C Barbey, M Robert, A Merieau, Y Konto-Ghiorghi, et al.. Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching. Molecular Plant-Microbe Interactions, 2019. ⟨hal-02356443⟩
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