Muscarinic receptor subtype mediating vasodilation feline middle cerebral artery exhibits M3 pharmacology. - Normandie Université Access content directly
Journal Articles European Journal of Pharmacology Year : 1990

Muscarinic receptor subtype mediating vasodilation feline middle cerebral artery exhibits M3 pharmacology.

Abstract

The nature of the muscarinic receptor subtype mediating the endothelium-dependent relaxation of the cat middle cerebral artery was investigated in vitro by recording the smooth muscle isometric tension of precontracted arterial segments. Relaxation induced by several agonists (acetylcholine (ACh), acetyl-beta-methylcholine, oxotremorine, carbachol and McN-A-343) was recorded. The ability of selective (pirenzepine, dicyclomine, adiphenine, AF-DX 116, methoctramine, gallamine, 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and hexahydro-sila-difenidol (HHSiD] and non-selective antagonists (atropine, scopolamine and quinuclidinyl benzilate (QNB] to block the relaxation induced by ACh was also estimated. The weak activity of the poorly selective M1 muscarinic receptor as together with the intermediate affinity of pirenzepine and adiphenine tend to exclude the M1 muscarinic receptor as the primary mediator of the cholinergic relaxation. The low affinity of AF-DX 116 and methoctramine further suggested that the cerebrovascular muscarinic receptor does not correspond to the M2 cardiac subtype. In contrast, 4-DAMP and HHSiD potently inhibited the ACh-induced relaxation with affinities similar to those reported at the M3 glandular receptor. We conclude that a similar to the pharmacological M3 muscarinic receptor subtype is responsible for the cholinergic relaxation of the cat middle cerebral artery.
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Dates and versions

hal-02135280 , version 1 (21-05-2019)

Identifiers

  • HAL Id : hal-02135280 , version 1
  • PUBMED : 2328761

Cite

François Dauphin, Edith Hamel. Muscarinic receptor subtype mediating vasodilation feline middle cerebral artery exhibits M3 pharmacology.. European Journal of Pharmacology, 1990, 178 (2), pp.203-13. ⟨hal-02135280⟩

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