Physico-chemical characterization and pharmacological activities of sulfated polysaccharide from sea urchin, Paracentrotus lividus - Normandie Université Access content directly
Journal Articles International Journal of Biological Macromolecules Year : 2017

Physico-chemical characterization and pharmacological activities of sulfated polysaccharide from sea urchin, Paracentrotus lividus

Abstract

Sulfated polysaccharide (SP) from the eggs of sea urchin Paracentrotus lividus, extracted by papain digestion, was characterized by size exclusion chromatography coupling on-line with light scattering and viscosity detectors (SEC/MALS/VD/DRI), gas chromatography coupled to mass spectrometer (GC–MS), and Fourier transform infrared spectroscopy (FTIR) analysis. The native molecular mass of the extracted polysaccharide is high (≥22 000 KDa) and it is composed mainly of arabinose, accompanied by other monosaccharides (mostly galactose, glucose and fucose), significant amounts of uronic acids (18.4%) and relatively high proportions of sulfate (22.4%). The pharmacological evaluation of SP showed a significant in vivo anti-inflammatory activity (p < 0.001), 3 h after injection, the edema inhibition was 75.8% at the dose of 100 mg/Kg; a significant peripheral analgesic activity (p < 0.001), with 64.9% of writhing inhibition, and a significant increase in the hot plate reaction time in mice indicating central analgesic activity. In addition, an interesting gastroprotective effect was observed with this polysaccharide; the gastric ulcer inhibition was 69.7%, at the dose of 100 mg/Kg.
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Dates and versions

hal-01869032 , version 1 (06-09-2018)

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Yosra Ben Salem, Safa Amri, Khaoula Mkadmini Hammi, Amal Abdelhamid, Didier Le Cerf, et al.. Physico-chemical characterization and pharmacological activities of sulfated polysaccharide from sea urchin, Paracentrotus lividus. International Journal of Biological Macromolecules, 2017, 97, pp.8 - 15. ⟨10.1016/j.ijbiomac.2017.01.007⟩. ⟨hal-01869032⟩
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