Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction - Normandie Université Access content directly
Journal Articles Marine drugs Year : 2014

Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction

Luc Picton
Lydia Rolland
  • Function : Author
Guy Potier
  • Function : Author

Abstract

Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.
Fichier principal
Vignette du fichier
marinedrugs-12-04851.pdf (718.39 Ko) Télécharger le fichier
Origin Publication funded by an institution

Dates and versions

hal-01866736 , version 1 (27-05-2021)

Licence

Identifiers

Cite

Pierre Saboural, Frederic Chaubet, François Rouzet, Faisal Al-Shoukr, Rana Azzouna, et al.. Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction. Marine drugs, 2014, 12 (9), pp.4851-4867. ⟨10.3390/md12094851⟩. ⟨hal-01866736⟩
101 View
156 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More