Analysis of Sugar Component of a Hot Water Extract from Arabidopsis thaliana Pollen Tubes Using GC-EI-MS
Résumé
Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 h-old Arabidopsis pollen tubes followed by a sugar composition analysis by gas chromatography mass spectrometry.
Domaines
Polymères Amélioration des plantes Biochimie [q-bio.BM] Biologie moléculaire Génomique, Transcriptomique et Protéomique [q-bio.GN] Réseaux moléculaires [q-bio.MN] Phytopathologie et phytopharmacie Biotechnologies Gamétogenèse Organisation et fonctions cellulaires [q-bio.SC] Interactions cellulaires [q-bio.CB]Origine | Fichiers produits par l'(les) auteur(s) |
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