Lipospermine-Mediated Gene Transfer Technique into Murine Cultured Cortical Cells. - Normandie Université Access content directly
Journal Articles Journal of Neuroscience Methods Year : 1997

Lipospermine-Mediated Gene Transfer Technique into Murine Cultured Cortical Cells.

Staedel C
  • Function : Author
Remy Js
  • Function : Author
Bahr A
  • Function : Author
Behr Jp
  • Function : Author

Abstract

In order to transfer exogenous DNA into embryonic cortical cells, we have chosen a transfection technique using a synthetic lipospermine (dipalmitoylphosphatidylethanolamylspermine, DPPES) which complexes DNA molecules and allows their penetration into the intracellular compartment. The procedure was optimized after testing several parameters: DPPES/DNA ratio, incubation time, kinetics of transgene expression, and growth medium. The protocol was achieved by following the expression of the E. coli LacZ reporter gene under the control of the cytomegalovirus promoter. The lipopolyamine-mediated transfection is efficient for terminally differentiated cells, since we routinely obtained transfection efficiencies of 30% for neurons.

Dates and versions

hal-01762050 , version 1 (09-04-2018)

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Brigitte Sola, Staedel C, Remy Js, Bahr A, Behr Jp. Lipospermine-Mediated Gene Transfer Technique into Murine Cultured Cortical Cells.. Journal of Neuroscience Methods, 1997, 71 (2), pp.183-186. ⟨10.1016/s0165-0270(96)00141-0⟩. ⟨hal-01762050⟩
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