Characterization of Two Signal Transduction Systems Involved in Intracellular Macrophage Survival and Environmental Stress Response in <i>Enterococcus faecalis</i> - Normandie Université Accéder directement au contenu
Article Dans Une Revue (Data Paper) Advances in Microbial Physiology Année : 2007

Characterization of Two Signal Transduction Systems Involved in Intracellular Macrophage Survival and Environmental Stress Response in Enterococcus faecalis

Résumé

The intracellular survival in mouse peritoneal macrophages of 8 Enterococcus faecalis response regulator mutants was tested to assess if the corresponding 2-component signal transduction systems (TCS) are involved in the ability of E. faecalis to survive in macrophages. Three mutants (err04, err05 and err06) are more susceptible than the wild-type JH2-2 strain and 1 is more resistant (err10). Then, characterization of the TCS Err04-Ehk04 and Err06-Ehk06 reveals that the first (homolog of PhoP-PhoR of Bacillus subtilis) is induced in phosphate deprivation conditions, regulates its own expression and plays a role in the expression of pstF encoding a phosphate-binding protein. The Err06-Ehk06 is involved in oxidative stress response. A mutation in the err06 gene increases sensitivity of the bacterium to H2O2. The err06-ehk06 operon is induced by H2O2 stress and controlled by 2 transcriptional start sites, of which 1 is specifically active in oxidative stress conditions. We also demonstrated that the expression of the catalase gene (kat) is partly dependant of the Err06-Ehk06 TCS.
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Dates et versions

hal-04158891 , version 1 (11-07-2023)

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Cécile Muller, Maurizio Sanguinetti, Eliette Riboulet, Laurent Hébert, Brunella Posteraro, et al.. Characterization of Two Signal Transduction Systems Involved in Intracellular Macrophage Survival and Environmental Stress Response in Enterococcus faecalis. Advances in Microbial Physiology, 2007, 14 (1-3), pp.59-66. ⟨10.1159/000106083⟩. ⟨hal-04158891⟩
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