Identification of the Enterococcus faecalis Tyrosine Decarboxylase Operon Involved in Tyramine Production - Normandie Université Accéder directement au contenu
Article Dans Une Revue (Data Paper) Applied and Environmental Microbiology Année : 2002

Identification of the Enterococcus faecalis Tyrosine Decarboxylase Operon Involved in Tyramine Production

Résumé

ABSTRACT Screening of a library of Enterococcus faecalis insertional mutants allowed isolation of a mutant affected in tyramine production. The growth of this mutant was similar to that of the wild-type E. faecalis JH2-2 strain in Maijala broth, whereas high-performance liquid chromatography analyses showed that tyramine production, which reached 1,000 μg ml −1 for the wild-type strain, was completely abolished. Genetic analysis of the insertion locus revealed a gene encoding a decarboxylase with similarity to eukaryotic tyrosine decarboxylases. Sequence analysis revealed a pyridoxal phosphate binding site, indicating that this enzyme belongs to the family of amino acid decarboxylases using this cofactor. Reverse transcription-PCR analyses demonstrated that the gene ( tdc ) encoding the putative tyrosine decarboxylase of E. faecalis JH2-2 is cotranscribed with the downstream gene encoding a putative tyrosine-tyramine antiporter and with the upstream tyrosyl-tRNA synthetase gene. This study is the first description of a tyrosine decarboxylase gene in prokaryotes.

Dates et versions

hal-04157250 , version 1 (10-07-2023)

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Nathalie Connil, Yoann Le Breton, Xavier Dousset, Yanick Auffray, Alain Rincé, et al.. Identification of the Enterococcus faecalis Tyrosine Decarboxylase Operon Involved in Tyramine Production. Applied and Environmental Microbiology, 2002, 68 (7), pp.3537-3544. ⟨10.1128/AEM.68.7.3537-3544.2002⟩. ⟨hal-04157250⟩
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