Identification and Characterization of gsp65 , an Organic Hydroperoxide Resistance ( ohr ) Gene Encoding a General Stress Protein in Enterococcus faecalis - Normandie Université Accéder directement au contenu
Article Dans Une Revue (Data Paper) Journal of Bacteriology Année : 2001

Identification and Characterization of gsp65 , an Organic Hydroperoxide Resistance ( ohr ) Gene Encoding a General Stress Protein in Enterococcus faecalis

Résumé

ABSTRACT The Enterococcus faecalis general stress protein Gsp65 has been purified from two-dimensional gel electrophoresis. Determination of its N-terminal sequence and characterization of the corresponding gene revealed that the gsp65 product is a 133-amino-acid protein sharing homologies with organic hydroperoxide resistance (Ohr) proteins. Transcriptional analysis of gsp65 gave evidence for a monocistronic mRNA initiated 52 nucleotides upstream of the ATG start codon and for an induction in response to hydrogen peroxide, heat shock, acid pH, detergents, ethanol, sodium chloride, and tert -butylhydroperoxide (tBOOH). A gsp65 mutant showed increased sensitivity to the organic hydroperoxide tBOOH and to ethanol.

Dates et versions

hal-04157210 , version 1 (10-07-2023)

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Alain Rincé, Jean-Christophe Giard, Vianney Pichereau, Sigrid Flahaut, Yanick Auffray. Identification and Characterization of gsp65 , an Organic Hydroperoxide Resistance ( ohr ) Gene Encoding a General Stress Protein in Enterococcus faecalis. Journal of Bacteriology, 2001, 183 (4), pp.1482-1488. ⟨10.1128/JB.183.4.1482-1488.2001⟩. ⟨hal-04157210⟩
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