Differentiated NHBE cells cultured in vitro at the air–liquid interface (ALI) were exposed to aerosolized indoor PM10 issued from a standard reference material (SRM 2585) using an aerosol-to-cell exposure system (Vitrocell® Cloud Max) that mimics more realistic exposure compared to traditional submerged conditions. The toxic effects of four daily repeated exposures to PM10 (0.1, 1 and 10 μg/cm2) were evaluated after the last day of exposure (day 4) and post-exposure (day 8 and day 11), in order to characterize delayed or transient responses. Our results have shown no significant modification in cytotoxicity, trans-epithelial electrical resistance and oxidative stress measurements, at any day of analysis. Limited inflammation response (IL-8) was noticed at day 4. Moreover, intrinsic reactive oxygen species production was characterized in acellular conditions together with endotoxins content. Therefore, the resistance of this relevant differentiated model associated with aerosol-to-cell exposures are discussed.