Effects of chemical compounds on the activity of the N‐Acetyl‐ β ‐D‐Glucosaminidase of the marine prawn, Palaemon serratus : screening in vitro . - Normandie Université Access content directly
Journal Articles Environmental Toxicology and Chemistry Year : 2023

Effects of chemical compounds on the activity of the N‐Acetyl‐ β ‐D‐Glucosaminidase of the marine prawn, Palaemon serratus : screening in vitro .

Abstract

N-acetyl-β-D-Glucosaminidase (NAGase) is important for crustaceans as the enzyme activity is necessary for the moulting process. The present study aimed to assess the sensitivity of the Palaemon serratus NAGase activity to a set of compounds of diverse chemical families, in a context of in vitro exposures. Compounds representing different chemical families were selected according to their abundance, impact in the environment, and their relevance as disruptors of the moulting process. In a first step, four solvents (DMSO, methanol, acetone, and ethanol) were tested to determine their suitability to dissolve hydrophobic compounds without affecting NAGase activity. Exclusively ethanol had no effect on enzyme activity and on the integrity of proteins present in the enzyme extract. Then, 18 other compounds were tested and six compounds, namely, pentoxifylline, fenoxycarb, dithiocarbamate, and RH5849 showed a specific alteration on the activity of NAGase, without affecting the protein content. However, cadmium, zinc, and glyphosate showed a nonspecific alteration, affecting both the enzyme activity and the proteins, whereas ibuprofen exclusively altered the protein content. Finally, ten of the 22 tested compounds (i.e. including DMSO, acetone and methanol) showed a direct alteration of NAGase activity.
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Dates and versions

hal-03959926 , version 1 (27-01-2023)

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Marc Rollin, Romain Coulaud, Béatrice Rocher, Elise Billoir, Olivier Geffard, et al.. Effects of chemical compounds on the activity of the N‐Acetyl‐ β ‐D‐Glucosaminidase of the marine prawn, Palaemon serratus : screening in vitro .. Environmental Toxicology and Chemistry, 2023, ⟨10.1002/etc.5567⟩. ⟨hal-03959926⟩
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