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Conference Papers Year : 2020

Identification of antiviral compounds against equid herpesvirus-1 using RTCA screening

Abstract

Equid alpha-herpesviruses (EHV) are responsible for different diseases in equine population. EHV-1 causes respiratory diseases, abortions and nervous disorders, In this presentation, we documented the interest of the Real-Time Cell Analysis (RTCA) technology to monitor the cytopathic effects induced by EHV-1 on equine dermal cells, and established the efficacy of this method to evaluate the antiviral effect of aciclovir and ganciclovir . In addition, the RTCA technology has also been found appropriate for the high-throughput screening of small molecules against EHV. Among the 2891 compounds screened, 22 molecules have been found to be effective in vitro against EHV-1. Decitabine, ganciclovir, and valganciclovir, were the most effective compounds identified, and their antiviral potency was further assessed on E. Derm, RK13 and EEK cells and against 3 different field strains of EHV-1 (ORF30 2254A/G/C) using RTCA technology. We also provide evidences of synergistic interactions between valganciclovir and decitabine in our in vitro antiviral assay as determined by impedance measurement and qPCR assays. (https://www.youtube.com/watch?v=haM3ThKw6GU)

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Dates and versions

hal-03219293 , version 1 (06-05-2021)

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  • HAL Id : hal-03219293 , version 1

Cite

Côme Thieulent, Stéphane Pronost, Gabrielle Sutton. Identification of antiviral compounds against equid herpesvirus-1 using RTCA screening. Labroots, Oct 2020, Caen, France. ⟨hal-03219293⟩
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