Communication Dans Un Congrès Année : 2014

Subcellular and subplastidial proteomics

Résumé

SUBCELLULAR AND SUBPLASTIDIAL PROTEOMICS Norbert Rolland1, Martino Tomizioli1, Cosmin Lazar2, Daniel Salvi1, Sabine Brugière2, Lucas Moyet1, Thomas Burger2, Giovanni Finazzi1, Daphné Berny1, Myriam Ferro2 1Laboratoire de Physiologie Cellulaire & Végétale. CNRS, CEA, INRA, Univ. Grenoble Alpes. CEA Grenoble, France; 2Laboratoire de Biologie à Grande Echelle. CEA, INSERM, Univ. Grenoble Alpes. CEA Grenoble, France; norbert.rolland@cea.fr Many high throughput proteomic experiments were conducted on purified cell organelles. We recently went a step further into the definition of the accurate localization of proteins within the chloroplast. We first performed a comprehensive analysis of the chloroplast proteome starting from the whole chloroplast and its purified three subcompartments (envelope, stroma and thylakoids) and assessed the partitioning of each protein in the 3 above-cited compartments (Ferro et al., Mol. Cell. Proteomics 2010). Using these data, we created the AT_CHLORO database (Bruley et al., Front. Plant Sci. 2012). In depth investigation of the subplastidial localization of identified proteins revealed new insights over chloroplast metabolism (Joyard et al., Mol. Plant 2009 & Prog. Lipid Res. 2010; Rolland et al., Annu. Rev. Genet. 2012). More recently, we performed new studies targeting the thylakoid subcompartments (Tomizioli et al., Mol. Cell. Proteomics 2014). These recent findings corroborate previous observations obtained for photosynthetic proteins that used nonproteomic approaches, and identify about a hundred minor thylakoid proteins being potential regulators of the chloroplast physiology.

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hal-04752551 , version 1 (24-10-2024)

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  • HAL Id : hal-04752551 , version 1

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Norbert Rolland, Martino Tomizioli, Cosmin Lazar, Daniel Salvi, Sabine Brugière, et al.. Subcellular and subplastidial proteomics. 1st INPPO World Congress on Plant Proteomics: Methodology to Biology, INPPO and University of Hamburg, Germany, Aug 2014, Hamburg (D), Germany. ⟨hal-04752551⟩
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