Effect of a biocontrol product in vitro and in planta against Phyphthora infestans in tomato - Normandie Université Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

Effect of a biocontrol product in vitro and in planta against Phyphthora infestans in tomato

Résumé

The Solanaceae crops, particularly potato and tomato, play a crucial role in global food production and are an important source of essential nutrients for human health. Indeed, they constitute the two most widely cultivated crops around the world, with a global production estimated, according by the FAO in 2020, at 388 and 182 million tonnes respectively. However, there are many biotic threats that can cause significant damage to these crops, leading to reduced their quality and yields. Late blight caused by the oomycete, Phytophthora infestans (Pi), represents one of the most devastating pathogens for both crops. To fight this pathogen, pesticides are frequently used, which can be harmful to the environment and public health. To minimize the use of phytopharmaceutical products, biocontrol agents are being investigated as potential alternatives for crops protection. This project aims to characterize the mode of action of GA342, a biocontrol product, developed by the company Gaïago, to improve its effectiveness against the late blight. In this context, the pathosystem Pi (36_A2 strain) - Micro-Tom, tomato model cultivar is used. Our first results showed that 1) GA342 has a direct effect on the growth of the oomycete. This effect led to decelerating or inhibiting totally the mycelial growth according the product’s dose. A characterization of the direct effects on sporangia, zoospores and mycelia is in progress. 2) in planta, the treatment with GA342 showed to reduce significantly the symptoms of the disease in a dose-dependent manner. We observed the efficacity of the product after 6 hours, 2 days and 8 days from its application. In order to explain the protective role of the product in planta, targeted transcriptomic (defense genes) analysis and biochemical approachs (callose and ROS) will also be established. The results related to the mode of action of GA342 will enable to enhance the effectiveness of this product for a better and efficient field application.
Fichier non déposé

Dates et versions

hal-04581748 , version 1 (21-05-2024)

Identifiants

  • HAL Id : hal-04581748 , version 1

Citer

Valentin Penaud, Abdelrahman Alahmad, Aude Bernardon-Mery, Karine Laval, Isabelle Trinsoutrot-Gattin, et al.. Effect of a biocontrol product in vitro and in planta against Phyphthora infestans in tomato. EAPR Pathology & Pests section meeting, inov3PT, Sep 2023, Arras, France. ⟨hal-04581748⟩
15 Consultations
0 Téléchargements

Partager

Gmail Mastodon Facebook X LinkedIn More