Diversity of nodule-inhabiting bacteria associated with cultivars of Pisum sativum and their biocontrol potential against Aphanomyces euteiches - Normandie Université Accéder directement au contenu
Poster De Conférence Année : 2023

Diversity of nodule-inhabiting bacteria associated with cultivars of Pisum sativum and their biocontrol potential against Aphanomyces euteiches

Résumé

Pea root rot, caused by the oomycete, Aphanomyces euteiches, is considered to be the most damaging pathology, as it can lead to significant losses in pea fields and there are currently no effective treatments for it. Studying plant microbiomes, such as bacterial endophytes present in pea nodules, can be a basis for valorizing them as potential biocontrol agents. For this purpose, nodules of three spring and three winter pea cultivars were investigated to isolate bacterial endophytes and test their potential biocontrol ability against A. euteiches. Moreover, a metabarcoding approach was performed to comprehensively assess the diversity of bacterial endophytes, with a particular emphasis on the biocontrol genera that are already described. Screening tests revealed 17 isolates, from five out of six cultivars, with an in vitro antagonist effect towards A. euteiches. High-throughput sequencing showed a predominance of Rhizobium lusitanum followed by Rhizobium leguminosarum in the nodules of all cultivars. High-throughput sequencing revealed a higher diversity of minor endophytes in the two winter cultivars, which are more resistant to frost, and known antagonist genera were more abundant in winter cultivars, making them better suited for biocontrol against A. euteiches. This research is the first to explore the microbiomes of nodule-inhabiting bacteria in multiple pea cultivars and provides a foundation for developing biocontrol strategies for managing pea root rot.
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Dates et versions

hal-04560944 , version 1 (26-04-2024)

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  • HAL Id : hal-04560944 , version 1

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Pauline Lusley, Adrien Gauthier, Isabelle Trinsoutrot-Gattin, Karine Laval, Mélanie Bressan. Diversity of nodule-inhabiting bacteria associated with cultivars of Pisum sativum and their biocontrol potential against Aphanomyces euteiches. 12th INTERNATIONAL CONGRESS OF PLANT PATHOLOGY, Aug 2023, Lyon, France. ⟨hal-04560944⟩
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