Skin biological responses to urban pollution in an ex vivo model
Résumé
The skin epidermis is continuously exposed to external aggressions, including environmental
pollution. The cosmetic industry must be able to offer dedicated products to fight the effects
of pollutants on the skin. We set up an experimental model that exposed skin explants
maintained in culture to a pollutant mixture. This mixture representing urban pollution was
designed on the basis of the French organization ‘Air Parif’ database. A chamber, called
Pollubox®, was built to allow a controlled nebulization of P on the cultured human skin
explants. We investigated ultrastructural morphology by transmission electron microscopy of
high pressure frozen skin explants. A global transcriptomic analysis indicated that the pollutant mixture was able to
induce relevant xenobiotic and antioxidant responses. Modulated detoxifying genes were
further investigated by laser micro-dissection coupled to qPCR, and immunochemistry. Both
approaches showed that P exposure correlated with overexpression of detoxifying genes and
provoked skin physiological alterations down to the stratum basale. The model developed
herein might be an efficient tool to study the effects of pollutants on skin as well as a
powerful testing method to evaluate the efficacy of cosmetic products against pollution.
Domaines
Biologie végétale Biologie cellulaire Amélioration des plantes Phytopathologie et phytopharmacie Génomique, Transcriptomique et Protéomique [q-bio.GN] Organisation et fonctions cellulaires [q-bio.SC] Interactions cellulaires [q-bio.CB] Biologie du développement Biochimie [q-bio.BM] Biologie moléculaire Biotechnologies
Fichier principal
Patatian-Delacour et al. revised main file.pdf (1.28 Mo)
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Patatian, Delacour et al. revised Supplemental Data.pdf (931.57 Ko)
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| Origine | Fichiers produits par l'(les) auteur(s) |
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| Origine | Fichiers produits par l'(les) auteur(s) |
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