<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02296503</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-26T18:43:41+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas</title>
            <author role="aut">
              <persName>
                <forename type="first">Sutin</forename>
                <surname>Kingtong</surname>
              </persName>
              <idno type="halauthorid">1658218-0</idno>
              <affiliation ref="#struct-267921"/>
              <affiliation ref="#struct-484746"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Kristell</forename>
                <surname>Kellner</surname>
              </persName>
              <email type="md5">247474d5af6b196f67b9a69629b6a800</email>
              <email type="domain">unicaen.fr</email>
              <idno type="idhal" notation="string">kristell-kellner</idno>
              <idno type="idhal" notation="numeric">184473</idno>
              <idno type="halauthorid" notation="string">17255-184473</idno>
              <idno type="IDREF">https://www.idref.fr/235516937</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-7343-2926</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=uJmsMGAAAAAJ</idno>
              <idno type="VIAF">https://viaf.org/viaf/85155767369127760603</idno>
              <affiliation ref="#struct-267921"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Benoît</forename>
                <surname>Bernay</surname>
              </persName>
              <idno type="halauthorid">201705-0</idno>
              <affiliation ref="#struct-527306"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Didier</forename>
                <surname>Goux</surname>
              </persName>
              <email type="md5">81d8d8cc4dbc189e325f95ede1820c7c</email>
              <email type="domain">unicaen.fr</email>
              <idno type="idhal" notation="numeric">1194379</idno>
              <idno type="halauthorid" notation="string">496602-1194379</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-2621-0386</idno>
              <affiliation ref="#struct-527306"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Pascal</forename>
                <surname>Sourdaine</surname>
              </persName>
              <email type="md5">9c2505277be17e502f7680a5e8abc914</email>
              <email type="domain">unicaen.fr</email>
              <idno type="idhal" notation="string">pascal-sourdaine</idno>
              <idno type="idhal" notation="numeric">739449</idno>
              <idno type="halauthorid" notation="string">10722-739449</idno>
              <idno type="IDREF">https://www.idref.fr/069283532</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9811-2876</idno>
              <affiliation ref="#struct-267921"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Clothilde Heude</forename>
                <surname>Berthelin</surname>
              </persName>
              <email type="md5">469830a74d8f6aaec0cff765a50ca42a</email>
              <email type="domain">unicaen.fr</email>
              <idno type="idhal" notation="string">clothilde-berthelin</idno>
              <idno type="idhal" notation="numeric">1512160</idno>
              <idno type="halauthorid" notation="string">1593165-1512160</idno>
              <idno type="ORCID">https://orcid.org/0009-0006-4246-7604</idno>
              <affiliation ref="#struct-267921"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Pascal</forename>
                <surname>SOURDAINE</surname>
              </persName>
              <email type="md5">9c2505277be17e502f7680a5e8abc914</email>
              <email type="domain">unicaen.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2019-09-25 11:39:56</date>
              <date type="whenModified">2026-03-13 16:12:55</date>
              <date type="whenReleased">2019-09-25 11:39:56</date>
              <date type="whenProduced">2013-04</date>
              <ref type="externalLink" target="https://api.istex.fr/ark:/67375/6H6-H0PG1HQ0-8/fulltext.pdf?sid=hal"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="16250">
                <persName>
                  <forename>Pascal</forename>
                  <surname>SOURDAINE</surname>
                </persName>
                <email type="md5">9c2505277be17e502f7680a5e8abc914</email>
                <email type="domain">unicaen.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02296503</idno>
            <idno type="halUri">https://normandie-univ.hal.science/hal-02296503</idno>
            <idno type="halBibtex">kingtong:hal-02296503</idno>
            <idno type="halRefHtml">&lt;i&gt;Journal of Proteomics&lt;/i&gt;, 2013, 82, pp.81-91. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.jprot.2013.02.009"&gt;&amp;#x27E8;10.1016/j.jprot.2013.02.009&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Journal of Proteomics, 2013, 82, pp.81-91. &amp;#x27E8;10.1016/j.jprot.2013.02.009&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="COMUE-NORMANDIE">Normandie Université</idno>
            <idno type="stamp" n="UNICAEN">Université de Caen Normandie</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Sutin</forename>
                    <surname>Kingtong</surname>
                  </persName>
                  <idno type="halauthorid">1658218-0</idno>
                  <affiliation ref="#struct-267921"/>
                  <affiliation ref="#struct-484746"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Kristell</forename>
                    <surname>Kellner</surname>
                  </persName>
                  <email type="md5">247474d5af6b196f67b9a69629b6a800</email>
                  <email type="domain">unicaen.fr</email>
                  <idno type="idhal" notation="string">kristell-kellner</idno>
                  <idno type="idhal" notation="numeric">184473</idno>
                  <idno type="halauthorid" notation="string">17255-184473</idno>
                  <idno type="IDREF">https://www.idref.fr/235516937</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-7343-2926</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=uJmsMGAAAAAJ</idno>
                  <idno type="VIAF">https://viaf.org/viaf/85155767369127760603</idno>
                  <affiliation ref="#struct-267921"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Benoît</forename>
                    <surname>Bernay</surname>
                  </persName>
                  <idno type="halauthorid">201705-0</idno>
                  <affiliation ref="#struct-527306"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Didier</forename>
                    <surname>Goux</surname>
                  </persName>
                  <email type="md5">81d8d8cc4dbc189e325f95ede1820c7c</email>
                  <email type="domain">unicaen.fr</email>
                  <idno type="idhal" notation="numeric">1194379</idno>
                  <idno type="halauthorid" notation="string">496602-1194379</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-2621-0386</idno>
                  <affiliation ref="#struct-527306"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Pascal</forename>
                    <surname>Sourdaine</surname>
                  </persName>
                  <email type="md5">9c2505277be17e502f7680a5e8abc914</email>
                  <email type="domain">unicaen.fr</email>
                  <idno type="idhal" notation="string">pascal-sourdaine</idno>
                  <idno type="idhal" notation="numeric">739449</idno>
                  <idno type="halauthorid" notation="string">10722-739449</idno>
                  <idno type="IDREF">https://www.idref.fr/069283532</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9811-2876</idno>
                  <affiliation ref="#struct-267921"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Clothilde Heude</forename>
                    <surname>Berthelin</surname>
                  </persName>
                  <email type="md5">469830a74d8f6aaec0cff765a50ca42a</email>
                  <email type="domain">unicaen.fr</email>
                  <idno type="idhal" notation="string">clothilde-berthelin</idno>
                  <idno type="idhal" notation="numeric">1512160</idno>
                  <idno type="halauthorid" notation="string">1593165-1512160</idno>
                  <idno type="ORCID">https://orcid.org/0009-0006-4246-7604</idno>
                  <affiliation ref="#struct-267921"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">60781</idno>
                <idno type="issn">1874-3919</idno>
                <idno type="eissn">1876-7737</idno>
                <title level="j">Journal of Proteomics</title>
                <imprint>
                  <publisher>Elsevier</publisher>
                  <biblScope unit="volume">82</biblScope>
                  <biblScope unit="pp">81-91</biblScope>
                  <date type="datePub">2013-04</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.jprot.2013.02.009</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Percoll gradient</term>
                <term xml:lang="en">Proteomics</term>
                <term xml:lang="en">Spermatozoa</term>
                <term xml:lang="en">Crassostrea gigas</term>
                <term xml:lang="en">Pacific oyster</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Knowledge of sperm maturation process is limited in the Pacific oyster Crassostrea gigas and major factors of fertilization success of this free spawning animal are unknown. We investigated proteins associated to spermatozoa by analyzing two cellular fractions obtained from a 40–80% Percoll gradient fractioning of germ cell of mature male gonads. Mature spermatozoa were enriched in the lower Percoll fraction while the upper fraction contained less mature or earlier germ cells. A 2-DE proteomic approach was used to identify differentially expressed proteins in both fractions. We screened out 31 differential proteins (P &lt; 0.05) which included 14 up-regulated and 17 down-regulated proteins. Using MALDI-TOF/TOF MS and bioinformatics search against a C. gigas database, 13 and 8 proteins were identified for the up-regulated and down-regulated groups, respectively. In the spermatozoa enriched fraction, proteins regarding flagellum formation and control, energy production and Proteosome subunit beta were increased. In less mature germ cell fraction, proteins regarding developmental processes and chaperon molecules were mainly increased. Our results improve current knowledge of proteins associated with spermatozoa maturation related to zootechnical practices used in mollusk hatcheries.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-267921" status="OLD">
          <idno type="IdRef">184555752</idno>
          <idno type="RNSR">201220076Z</idno>
          <orgName>Biologie des mollusques marins et des écosystèmes associés</orgName>
          <orgName type="acronym">BioMEA</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2013-12-31</date>
          <desc>
            <address>
              <addrLine>Esplanade de la paix 14032 CAEN CEDEX 5</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-7127" type="direct"/>
            <relation active="#struct-455934" type="indirect"/>
            <relation name="FRE3484" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-484746" status="VALID">
          <idno type="ISNI">0000 0000 9482 780X</idno>
          <idno type="ROR">https://ror.org/01ff74m36</idno>
          <orgName>Burapha University</orgName>
          <orgName type="acronym">BU</orgName>
          <desc>
            <address>
              <addrLine>169 Longhaad Bangsaen Road, Saensook, Mueang, ChonBuri 20131</addrLine>
              <country key="TH"/>
            </address>
            <ref type="url">http://www.buu.ac.th/</ref>
          </desc>
        </org>
        <org type="regrouplaboratory" xml:id="struct-527306" status="OLD">
          <idno type="RNSR">200721305G</idno>
          <orgName>Interactions Cellules Organismes Environnement</orgName>
          <orgName type="acronym">ICORE</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2021-12-31</date>
          <desc>
            <address>
              <addrLine>Esplanade de la Paix CS 14032 14032 CAEN Cedex 5</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://icore.unicaen.fr/</ref>
          </desc>
          <listRelation>
            <relation name="SF 4206" active="#struct-7127" type="direct"/>
            <relation active="#struct-455934" type="indirect"/>
            <relation active="#struct-300185" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-7127" status="VALID">
          <idno type="IdRef">026403064</idno>
          <idno type="ISNI">0000000121864076</idno>
          <idno type="ROR">https://ror.org/051kpcy16</idno>
          <orgName>Université de Caen Normandie</orgName>
          <orgName type="acronym">UNICAEN</orgName>
          <date type="start">1432-01-01</date>
          <desc>
            <address>
              <addrLine>Esplanade de la Paix - CS 14032 - 14032 CAEN Cedex 5</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.unicaen.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-455934" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-455934" status="VALID">
          <idno type="IdRef">190906332</idno>
          <idno type="ISNI">0000000417859671 </idno>
          <idno type="ROR">https://ror.org/01k40cz91</idno>
          <orgName>Normandie Université</orgName>
          <orgName type="acronym">NU</orgName>
          <date type="start">2015-01-01</date>
          <desc>
            <address>
              <addrLine>Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.normandie-univ.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300185" status="VALID">
          <idno type="IdRef">031245382</idno>
          <idno type="ISNI">0000000404720160</idno>
          <idno type="ROR">https://ror.org/027arzy69</idno>
          <idno type="Wikidata">Q2945740</idno>
          <orgName>CHU Caen Normandie – Centre Hospitalier Universitaire de Caen Normandie</orgName>
          <orgName type="acronym">CHU Caen Normandie</orgName>
          <desc>
            <address>
              <addrLine>Avenue de la Côte de Nacre 14000 Caen</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.chu-caen.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-455934" type="direct"/>
          </listRelation>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>