<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02268975</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-26T18:49:12+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Genetic and pharmacological inactivation of d-alanylation of teichoic acids sensitizes pathogenic enterococci to β-lactams</title>
            <author role="aut">
              <persName>
                <forename type="first">Delphine</forename>
                <surname>Coupri</surname>
              </persName>
              <idno type="halauthorid">1638278-0</idno>
              <affiliation ref="#struct-257103"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Aurélie</forename>
                <surname>Budin-Verneuil</surname>
              </persName>
              <idno type="halauthorid">442007-0</idno>
              <affiliation ref="#struct-257103"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Axel</forename>
                <surname>Hartke</surname>
              </persName>
              <idno type="halauthorid">219466-0</idno>
              <affiliation ref="#struct-257103"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Abdellah</forename>
                <surname>Benachour</surname>
              </persName>
              <email type="md5">edb37f4ed367193e8638fb9b8aaad663</email>
              <email type="domain">unicaen.fr</email>
              <idno type="idhal" notation="numeric">867137</idno>
              <idno type="halauthorid" notation="string">442012-867137</idno>
              <affiliation ref="#struct-257103"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Loïc</forename>
                <surname>Léger</surname>
              </persName>
              <idno type="halauthorid">1638279-0</idno>
              <affiliation ref="#struct-257103"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Thierry</forename>
                <surname>Lequeux</surname>
              </persName>
              <email type="md5">bd56b21e98093aa8b6a05ddac55901e0</email>
              <email type="domain">ensicaen.fr</email>
              <idno type="idhal" notation="string">thierrylequeuxlcmt</idno>
              <idno type="idhal" notation="numeric">959682</idno>
              <idno type="halauthorid" notation="string">609195-959682</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3783-4458</idno>
              <idno type="IDREF">https://www.idref.fr/114247196</idno>
              <affiliation ref="#struct-901"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Emmanuel</forename>
                <surname>Pfund</surname>
              </persName>
              <email type="md5">f765910717e3a60fe17079bf178bcdd2</email>
              <email type="domain">ensicaen.fr</email>
              <idno type="idhal" notation="numeric">765468</idno>
              <idno type="halauthorid" notation="string">609193-765468</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-1896-0109</idno>
              <idno type="IDREF">https://www.idref.fr/06997411X</idno>
              <affiliation ref="#struct-901"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nicolas</forename>
                <surname>Verneuil</surname>
              </persName>
              <email type="md5">2ff67eb0bb0a341311801dbb45189287</email>
              <email type="domain">unicaen.fr</email>
              <idno type="idhal" notation="string">nicolas-verneuil</idno>
              <idno type="idhal" notation="numeric">1672732</idno>
              <idno type="halauthorid" notation="string">765849-1672732</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-9080-6627</idno>
              <affiliation ref="#struct-257103"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Axel</forename>
                <surname>Hartke</surname>
              </persName>
              <email type="md5">f9110c1aa4e9aaa966e4c7be8c227eb9</email>
              <email type="domain">unicaen.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2019-08-22 08:48:04</date>
              <date type="whenModified">2026-04-23 11:44:40</date>
              <date type="whenReleased">2019-08-22 08:48:04</date>
              <date type="whenProduced">2019-07-24</date>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="754941">
                <persName>
                  <forename>Axel</forename>
                  <surname>Hartke</surname>
                </persName>
                <email type="md5">f9110c1aa4e9aaa966e4c7be8c227eb9</email>
                <email type="domain">unicaen.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02268975</idno>
            <idno type="halUri">https://normandie-univ.hal.science/hal-02268975</idno>
            <idno type="halBibtex">coupri:hal-02268975</idno>
            <idno type="halRefHtml">&lt;i&gt;Journal of Antimicrobial Chemotherapy&lt;/i&gt;, 2019, 74 (11), pp.3162-3169. &lt;a target="_blank" href="https://dx.doi.org/10.1093/jac/dkz322"&gt;&amp;#x27E8;10.1093/jac/dkz322&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Journal of Antimicrobial Chemotherapy, 2019, 74 (11), pp.3162-3169. &amp;#x27E8;10.1093/jac/dkz322&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INSA-ROUEN">Institut National des Sciences Appliquées de Rouen</idno>
            <idno type="stamp" n="ENSI-CAEN">Ecole Nationale Supérieure d'Ingénieurs de Caen</idno>
            <idno type="stamp" n="COMUE-NORMANDIE">Normandie Université</idno>
            <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno>
            <idno type="stamp" n="UNIROUEN">Université de Rouen Normandie</idno>
            <idno type="stamp" n="ENSICAEN">École Nationale Supérieure d’Ingénieurs de Caen</idno>
            <idno type="stamp" n="UNILEHAVRE">Université Le Havre Normandie</idno>
            <idno type="stamp" n="UNICAEN">Université de Caen Normandie</idno>
            <idno type="stamp" n="LCMT">Laboratoire de chimie moléculaire et thioorganique</idno>
            <idno type="stamp" n="INSA-GROUPE">Groupe INSA</idno>
            <idno type="stamp" n="TEST-HALCNRS">Collection test HAL CNRS</idno>
            <idno type="stamp" n="TEST2-HALCNRS">TEST2-HALCNRS</idno>
            <idno type="stamp" n="LABOS-XL-CHEM">École Universitaire de Recherche (EUR) XL-Chem</idno>
            <idno type="stamp" n="LABOS-SYNORG">Labex SYNORG</idno>
            <idno type="stamp" n="CBSA-GLOBAL">CBSA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Genetic and pharmacological inactivation of d-alanylation of teichoic acids sensitizes pathogenic enterococci to β-lactams</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Delphine</forename>
                    <surname>Coupri</surname>
                  </persName>
                  <idno type="halauthorid">1638278-0</idno>
                  <affiliation ref="#struct-257103"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Aurélie</forename>
                    <surname>Budin-Verneuil</surname>
                  </persName>
                  <idno type="halauthorid">442007-0</idno>
                  <affiliation ref="#struct-257103"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Axel</forename>
                    <surname>Hartke</surname>
                  </persName>
                  <idno type="halauthorid">219466-0</idno>
                  <affiliation ref="#struct-257103"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Abdellah</forename>
                    <surname>Benachour</surname>
                  </persName>
                  <email type="md5">edb37f4ed367193e8638fb9b8aaad663</email>
                  <email type="domain">unicaen.fr</email>
                  <idno type="idhal" notation="numeric">867137</idno>
                  <idno type="halauthorid" notation="string">442012-867137</idno>
                  <affiliation ref="#struct-257103"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Loïc</forename>
                    <surname>Léger</surname>
                  </persName>
                  <idno type="halauthorid">1638279-0</idno>
                  <affiliation ref="#struct-257103"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Thierry</forename>
                    <surname>Lequeux</surname>
                  </persName>
                  <email type="md5">bd56b21e98093aa8b6a05ddac55901e0</email>
                  <email type="domain">ensicaen.fr</email>
                  <idno type="idhal" notation="string">thierrylequeuxlcmt</idno>
                  <idno type="idhal" notation="numeric">959682</idno>
                  <idno type="halauthorid" notation="string">609195-959682</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3783-4458</idno>
                  <idno type="IDREF">https://www.idref.fr/114247196</idno>
                  <affiliation ref="#struct-901"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Emmanuel</forename>
                    <surname>Pfund</surname>
                  </persName>
                  <email type="md5">f765910717e3a60fe17079bf178bcdd2</email>
                  <email type="domain">ensicaen.fr</email>
                  <idno type="idhal" notation="numeric">765468</idno>
                  <idno type="halauthorid" notation="string">609193-765468</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-1896-0109</idno>
                  <idno type="IDREF">https://www.idref.fr/06997411X</idno>
                  <affiliation ref="#struct-901"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nicolas</forename>
                    <surname>Verneuil</surname>
                  </persName>
                  <email type="md5">2ff67eb0bb0a341311801dbb45189287</email>
                  <email type="domain">unicaen.fr</email>
                  <idno type="idhal" notation="string">nicolas-verneuil</idno>
                  <idno type="idhal" notation="numeric">1672732</idno>
                  <idno type="halauthorid" notation="string">765849-1672732</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-9080-6627</idno>
                  <affiliation ref="#struct-257103"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">15068</idno>
                <idno type="issn">0305-7453</idno>
                <idno type="eissn">1460-2091</idno>
                <title level="j">Journal of Antimicrobial Chemotherapy</title>
                <imprint>
                  <publisher>Oxford University Press (OUP)</publisher>
                  <biblScope unit="volume">74</biblScope>
                  <biblScope unit="issue">11</biblScope>
                  <biblScope unit="pp">3162-3169</biblScope>
                  <date type="datePub">2019-07-24</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1093/jac/dkz322</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halDomain" n="sdv.bbm">Life Sciences [q-bio]/Biochemistry, Molecular Biology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Background :Enterococci intrinsically resistant to cephalosporins represent a major cause of healthcare-associated infections, and the emergence of MDR makes therapeutic approaches particularly challenging.Objectives :Teichoic acids are cell wall glycopolymers present in Gram-positive bacteria. Teichoic acids can be modified by D-alanylation, which requires four proteins encoded by the dltABCD operon. Our objective was to evaluate the Dlt system as a druggable target to treat enterococcal infections.Methods :The susceptibility of a D-alanylation-deficient strain of Enterococcus faecalis to β-lactam antibiotics individually and/or in combination was analysed. Moreover, a DltA inhibitor was synthesized to test pharmacological inhibition of D-alanylation in vivo and in host using the animal model Galleria mellonella with different clinical isolates of E. faecalis and Enterococcus faecium.Results :Most cephalosporins used as mono treatment had no impact on survival of the parental strain, but were slightly lethal for the dltA mutant of E. faecalis. Addition of a very low concentration of amoxicillin significantly increased killing of the dltA mutant under these conditions. The most spectacular effect was obtained with a combination of cefotaxime (1 mg/L) and amoxicillin (0.03 mg/L). In the presence of the inhibitor, the WT strain was as susceptible to this combination treatment as the dltA mutant. This molecule associated with the antibiotics was also effective in killing other E. faecalis clinical isolates and successfully prevented death of Galleria infected with either E. faecalis or E. faecium.Conclusions :The combined results support the potential usefulness of the Dlt system as a target to potentiate antibiotic combination therapies for the treatment of drug-resistant enterococci.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-257103" status="OLD">
          <idno type="IdRef">199281084</idno>
          <idno type="RNSR">201220075Y</idno>
          <orgName>Unité de Recherche Risques Microbiens</orgName>
          <orgName type="acronym">U2RM</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2021-12-31</date>
          <desc>
            <address>
              <addrLine>Esplanade de la paix - CS 14032 14032 Caen cedex 5</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://recherche.unicaen.fr/ea-4655-ur-risques-microbiens-u2rm--283407.kjsp</ref>
          </desc>
          <listRelation>
            <relation name="EA 4655" active="#struct-7127" type="direct"/>
            <relation active="#struct-455934" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-901" status="OLD">
          <idno type="IdRef">184088429</idno>
          <idno type="ISNI">0000 0004 0386 0348</idno>
          <idno type="RNSR">199612302U</idno>
          <idno type="ROR">https://ror.org/03q7p3t30</idno>
          <idno type="Wikidata">Q30262276</idno>
          <orgName>Laboratoire de chimie moléculaire et thioorganique</orgName>
          <orgName type="acronym">LCMT</orgName>
          <date type="start">1996-01-01</date>
          <date type="end">2024-12-31</date>
          <desc>
            <address>
              <addrLine>Bât. B 6, Bvd du Maréchal Juin 14050 CAEN CEDEX 4</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.lcmt.ensicaen.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-7127" type="direct"/>
            <relation active="#struct-455934" type="indirect"/>
            <relation active="#struct-246955" type="direct"/>
            <relation active="#struct-302261" type="direct"/>
            <relation active="#struct-300317" type="indirect"/>
            <relation active="#struct-300318" type="indirect"/>
            <relation active="#struct-301288" type="indirect"/>
            <relation active="#struct-301232" type="indirect"/>
            <relation active="#struct-341038" type="direct"/>
            <relation name="FR3038" active="#struct-441569" type="direct"/>
            <relation name="UMR6507" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-7127" status="VALID">
          <idno type="IdRef">026403064</idno>
          <idno type="ISNI">0000000121864076</idno>
          <idno type="ROR">https://ror.org/051kpcy16</idno>
          <orgName>Université de Caen Normandie</orgName>
          <orgName type="acronym">UNICAEN</orgName>
          <date type="start">1432-01-01</date>
          <desc>
            <address>
              <addrLine>Esplanade de la Paix - CS 14032 - 14032 CAEN Cedex 5</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.unicaen.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-455934" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-455934" status="VALID">
          <idno type="IdRef">190906332</idno>
          <idno type="ISNI">0000000417859671 </idno>
          <idno type="ROR">https://ror.org/01k40cz91</idno>
          <orgName>Normandie Université</orgName>
          <orgName type="acronym">NU</orgName>
          <date type="start">2015-01-01</date>
          <desc>
            <address>
              <addrLine>Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.normandie-univ.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-246955" status="VALID">
          <idno type="IdRef">190907991</idno>
          <idno type="ROR">https://ror.org/01fpqqe90</idno>
          <orgName>École Nationale Supérieure d'Ingénieurs de Caen</orgName>
          <orgName type="acronym">ENSICAEN</orgName>
          <desc>
            <address>
              <addrLine>6, Boulevard du Maréchal Juin CS 45053 14050 CAEN cedex 04</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ensicaen.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-455934" type="direct"/>
          </listRelation>
        </org>
        <org type="regrouplaboratory" xml:id="struct-302261" status="VALID">
          <idno type="RNSR">200810697X</idno>
          <orgName>Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire</orgName>
          <orgName type="acronym">INC3M</orgName>
          <date type="start">2008-01-01</date>
          <desc>
            <address>
              <addrLine>Rue Tesnière76821 MONT ST AIGNAN CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.lcmt.ensicaen.fr/federation-inc3m/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-7127" type="direct"/>
            <relation active="#struct-455934" type="indirect"/>
            <relation active="#struct-246955" type="direct"/>
            <relation active="#struct-300317" type="direct"/>
            <relation active="#struct-300318" type="direct"/>
            <relation active="#struct-301288" type="direct"/>
            <relation active="#struct-301232" type="indirect"/>
            <relation active="#struct-341038" type="direct"/>
            <relation name="FR3038" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300317" status="VALID">
          <idno type="IdRef">031308570</idno>
          <idno type="ISNI">0000000121731046</idno>
          <idno type="ROR">https://ror.org/05v509s40</idno>
          <orgName>Université Le Havre Normandie</orgName>
          <orgName type="acronym">ULH</orgName>
          <date type="start">1984-08-27</date>
          <desc>
            <address>
              <addrLine>25 rue Philippe Lebon - BP 1123 - 76063 Le Havre Cedex France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.univ-lehavre.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-455934" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300318" status="VALID">
          <idno type="IdRef">026403919</idno>
          <idno type="ISNI">0000000121083034</idno>
          <idno type="ROR">https://ror.org/03nhjew95</idno>
          <orgName>Université de Rouen Normandie</orgName>
          <orgName type="acronym">UNIROUEN</orgName>
          <date type="start">1966-01-01</date>
          <desc>
            <address>
              <addrLine>1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-rouen.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-455934" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-301288" status="VALID">
          <idno type="IdRef">033364346</idno>
          <idno type="ISNI">0000000121908462</idno>
          <idno type="ROR">https://ror.org/020ws7586</idno>
          <orgName>Institut national des sciences appliquées Rouen Normandie</orgName>
          <orgName type="acronym">INSA Rouen Normandie</orgName>
          <date type="start">1985-01-01</date>
          <desc>
            <address>
              <addrLine>Avenue de l’Université 76801 Saint-Étienne-du-Rouvray Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.insa-rouen.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301232" type="direct"/>
            <relation active="#struct-455934" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-301232" status="VALID">
          <idno type="IdRef">162105150</idno>
          <orgName>Institut National des Sciences Appliquées</orgName>
          <orgName type="acronym">INSA</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-341038" status="VALID">
          <idno type="IdRef">232435103</idno>
          <idno type="ISNI">000000010626358X</idno>
          <idno type="ROR">https://ror.org/02cte4b68</idno>
          <orgName>Institut de Chimie - CNRS Chimie</orgName>
          <orgName type="acronym">INC-CNRS</orgName>
          <date type="start">2009-10-01</date>
          <desc>
            <address>
              <addrLine>CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE3 rue Michel-Ange75794 PARIS CEDEX 16 - France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cnrs.fr/inc</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>