GABA inhibits endozepine release from cultured rat astrocytes - Normandie Université Access content directly
Journal Articles Glia Year : 1999

GABA inhibits endozepine release from cultured rat astrocytes

Abstract

In the mammalian brain, the endogenous ligands for benzodiazepine receptors (also called endozepines) are predominantly synthesized by glial cells. It has recently been reported that rat astrocytes in primary culture release substantial amounts of endozepines. The aim of the present study was to investigate the possible involvement of GABA in the control of endozepine release. Exposure of cultured rat astrocytes to GABA (10(-7) to 10(-5) M) induced a dose-related inhibition of endozepine secretion. At higher doses (3 x 10(-5) to 10(-3) M), the effect of GABA gradually diminished. The inhibitory effect of GABA (10(-5) M) was mimicked by the GABA(B) receptor agonist baclofen (10(-5) M). In contrast, the GABA(A) receptor agonists 3APS and isoguvacine (10(-5) M each) did not modify endozepine release. The inhibition of endozepine secretion evoked by GABA and baclofen (10(-5) M each) was totally abrogated by the specific GABA(B) receptor antagonist phaclofen (10(-4) M). GABA and baclofen caused a significant inhibition of forskolin-evoked production of cAMP in astrocytes and this effect was abolished in the presence of phaclofen. In contrast, isoguvacine had no effect on cAMP production. Exposure of astrocytes to dbcAMP induced a time- and dose-dependent stimulation of endozepine release. These data indicate that GABA, acting through GABA(B) receptors negatively coupled to adenylyl cyclase, inhibits endozepine release from cultured rat astrocytes. The secretion of endozepines thus appears to be a valuable marker to monitor astrocyte activity.

Dates and versions

hal-01973857 , version 1 (08-01-2019)

Identifiers

Cite

Christine Patte, Pierrick Gandolfo, Jérôme Leprince, Jean-Louis Thoumas, Marc Fontaine, et al.. GABA inhibits endozepine release from cultured rat astrocytes. Glia, 1999, 25 (4), pp.404-411. ⟨10.1002/(SICI)1098-1136(19990215)25:4<404::AID-GLIA9>3.0.CO;2-Q⟩. ⟨hal-01973857⟩
55 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More