Molecular PhylogenyandUltrastructure of Aphelidium aff.melosirae (Aphelida, Opisthosporidia) - Université Pierre et Marie Curie
Journal Articles Protist Year : 2014

Molecular PhylogenyandUltrastructure of Aphelidium aff.melosirae (Aphelida, Opisthosporidia)

Abstract

Aphelids areapoorlyknowngroupofparasitoidsofalgaethathaveraisedconsiderableinterestdueto their pivotalphylogeneticposition.TogetherwithCryptomycotaandthehighlyderivedMicrosporidia, theyhavebeenrecentlyre-classifiedasOpisthosporidia,beingthesistergrouptofungi.Despitetheir hugediversity,asrevealed by molecular environmentalstudies,andtheirphylogeneticinterest,only three generahavebeendescribed(Aphelidium, Amoeboaphelidium, and Pseudaphelidium), fromwhich 18S rRNAgenesequencesexistonly for Amoeboaphelidium species. Here,wedescribethelifecycle and ultrastructureof Aphelidium aff. melosirae, andprovidethefirst18SrRNAgenesequenceobtained for this genus.Molecularphylogenyanalysisindicatesthat Aphelidium is verydistantlyrelatedto Amoebaphelidium, highlightingthewidegeneticdiversityoftheaphelids.Theparasitoidencystsand penetrates thehostalga, Tribonemagayanum throughaninfectiontube.Cystgerminationleadsto a youngtrophontthatphagocytesthealgalcellcontentandprogressivelydevelopsaplasmodium, whichbecomesazoospore-producingsporangium. Aphelidium aff. melosirae has amoeboflagellate zoospores, tubular/lamellarmitochondrialcristae,ametazoantypeofcentrosome,andclosedorthomi- tosis withanintranuclearspindle.Thesefeaturestogetherwithtrophontphagocytosisdistinguish Aphelidium fromfungiandsupporttheerectionofthe new superphylumOpisthosporidiaassisterto fungi.
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Dates and versions

hal-01049981 , version 1 (03-11-2024)

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Sergey A. Karpov, Maria A. Mamkaeva, Karim Benzerara, David Moreira, Purificación López-García. Molecular PhylogenyandUltrastructure of Aphelidium aff.melosirae (Aphelida, Opisthosporidia). Protist, 2014, 165, pp.512-526. ⟨10.1016/j.protis.2014.05.003⟩. ⟨hal-01049981⟩
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